Pluriselect/Lympho Spin Medium / PBMC Spin Medium/60-00092/100 ml,Pluriselect,,Pluriselect,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Pluriselect/Lympho Spin Medium / PBMC Spin Medium/60-00092/100 ml

产品编号：60-00092

市场价：¥480.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞培养>培养基>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$24.00

品牌： Pluriselect

公司：Pluriselect

公司分类：

商品介绍

Details:

LymphoSpinMediumisareadytouse,steriledensitygrADIentmediumfortheisolationofmononuclearcells(PBMC)inhighyieldfromfreshperipheralwholebloodandbuffycoatbyasimplecentrifugationprocedure.Mononuclearcellscanbeusedforawiderangeofdownstreamapplicationssuchasmagneticactivatedcellsorting(suchasMACS¹)orstemcellresearch.

LymphoSpinMediumcanbeusedassubstituteforFicoll™^,2andLymphoprep™^,3withoutanyneedtochangetheexistingprotocols.

SeparationschemeforPBMCenrichmentwithPBMCSpinMedium

AtfirstcarefullylayerthesamplematerialontopofthePBMCSpinMedium.Avoidamixingofthetwophases.Alternativelyit’spossIBLetousepluriMatetubes.Thebarrierpreventsthemixingofthetwophasesandenablestopourofftheenrichedcellsafterthefirstcentrifugationstep.Afterthedensitycentrifugationaspiratetheupperlayer(Plasmaanddilutionbuffer).Afterwardstransferthemononuclearcelllayertoanewconicaltubeandwashthecells.

PBMC preparation with Lympho Spin Medium. The sample material will be overlayed on top of the density medium.

Fig.1:PreparationofPBMCwithLymphoSpinMedium.Thesamplematerialwillbeoverlayedontopofdensitygradientmedium.

Whole blood after density gradient centrifugation with Lympho Spin. The PBMC can be found on top of the Lympho Spin Medium.

Fig.2.:Layersafterdensitygradientcentrifugation.ThePBMCcanbefoundontopoftheLymphoSpinMedium.Erythrocytes,Granulocytesanddeadcellswillpassthemediumandcanbefoundatthebottomofthetube.

¹MACSisatrademarkofMiltenyiBiotecGmbH;²Ficoll™isatrademarkofGEHealthcare;³Lymphoprep™isatrademarkofAxis-Shield/Alere

MoreInformation:

SampleMaterial	WholeBlood,PBMC,BuffyCoat,CordBlood,BoneMarrow,PrimaryCellSolution
StorageCondition	RoomTemperature
RegulatoryStatement	Forresearchuseonly.Notforuseindiagnosticprocedures,

品牌介绍

Pluriselect

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Welcome to pluriSelect, your worldwide supplier of products optimized for cell and protein separation for the research in the fields of immunology, basic science and diagnostics. Our main aim is to supply you with high quality products and best possible service, ensuring your experiments are free of hassle from the shopping experience to the finish of your experimental work.

We want to support your vision to contribute the challenging task of finding new therapies for autoimmune diseases such as rheumatic illnesses, arteriosclerosis and allergies, as well as simplifying the isolation of circulating tumor cells (CTC).

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